interleukin il 12p70  (R&D Systems)

Journal: NPJ Vaccines

Article Title: Toxoplasma WH3 Δ rop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis

doi: 10.1038/s41541-024-00996-9

Figure Lengend Snippet: Detection of cytokines and T. gondii -specific IgG level in the sera of mice at 30/75/125 dpv with WH3 Δ rop18 by ELISA test. a Strategy diagram of the experimental process. b IFN-γ, c IL-12p70, d TNF-α, e IL-10 ( n = 5) and f Toxoplasma -specific total IgG and IgG1 and IgG2a ( n = 8). Serum was derived from unvaccinated mice as a negative control. *** P < 0.001, ** P < 0.01, * P < 0.05, ns not significant.

Article Snippet: In the meantime, the expression levels of cytokines interferon-gamma (IFN-γ), interleukin 12p70 (IL-12p70), tumor necrosis factor TNF (TNF-α) and interleukin 10 (IL-10) were detected by using ELISA kits (ProteinTech Group, Inc., USA) according to the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Negative Control

Journal: NPJ Vaccines

Article Title: Toxoplasma WH3 Δ rop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis

doi: 10.1038/s41541-024-00996-9

Figure Lengend Snippet: a Schematic illustration of the experimental procedure. b , d Percentage of CD3 + CD8 + T cells and CD3 + CD4 + T cells detected with FCM ( n = 4). c , e Increased percentage of Th1 (CD4 + IFN-γ + ) cell population in the mouse spleens following WH3 Δ rop18 immunization compared to the control ( n = 4). f Spleen cells cultured in vitro were stimulated with 10 µg/mL WH3 WT soluble antigens. Subsequently, the levels of IFN-γ, TNF-α, IL-12p70 and IL-10 in the splenic cell culture supernatant were detected by ELISA ( n = 5). Unpaired t -tests were used for statistical analysis. Bars = mean ± SEM. * P < 0.05, *** P < 0.001.

Article Snippet: In the meantime, the expression levels of cytokines interferon-gamma (IFN-γ), interleukin 12p70 (IL-12p70), tumor necrosis factor TNF (TNF-α) and interleukin 10 (IL-10) were detected by using ELISA kits (ProteinTech Group, Inc., USA) according to the manufacturer’s recommendations.

Techniques: Control, Cell Culture, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: NPJ Vaccines

Article Title: Toxoplasma WH3 Δ rop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis

doi: 10.1038/s41541-024-00996-9

Figure Lengend Snippet: 10 3 WH3 Δ rop18 parasites were i.p. into BALB/c mice for 75 days as a vaccine dose. Then, the mice were challenged with 10 3 WH3 WT or 10 5 WH6 tachyzoites. Unvaccinated mice were treated similarly. a Schematic illustration of the study design. b The vaccinated mice were challenged with 10 3 WH3 WT tachyzoites or c 10 5 WH6 tachyzoites. The survival rate was recorded for 35 days ( n = 10). Gehan-Breslow-Wilcoxon tests. d WH3 WT or WH6 tachyzoites were used for challenges of vaccinated mice, and 7 days later, the parasite burden in ascites were tested by qPCR ( n = 5). *** P < 0.001. e IFN-γ, IL-12p70, TNF-α and IL-10 in sera were tested by ELISA ( n = 5), *** P < 0.001. f , g Toxoplasma -specific total IgG and subclasses of IgG1 and IgG2a in sera were tested by ELISA. Unpaired t -tests were used for s t atistical analysis. Bars = mean ± SEM. *** P < 0.001.

Article Snippet: In the meantime, the expression levels of cytokines interferon-gamma (IFN-γ), interleukin 12p70 (IL-12p70), tumor necrosis factor TNF (TNF-α) and interleukin 10 (IL-10) were detected by using ELISA kits (ProteinTech Group, Inc., USA) according to the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: NPJ Vaccines

Article Title: Toxoplasma WH3 Δ rop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis

doi: 10.1038/s41541-024-00996-9

Figure Lengend Snippet: 10 3 WH3 Δ rop18 parasites were i.p. into BALB/c mice as a vaccine dose and observed for 125 days. Vaccinated or un-vaccinated mice were then infected with 10 3 RH or 10 5 ME49 parasites. a Schematic illustration of the study design. b 10 3 RH or c 10 5 ME49 tachyzoites were used for challenges ( n = 10). The survival rate was recorded for 35 days. Gehan-Breslow-Wilcoxon tests. d RH or ME49 tachyzoites challenges in vaccinated mice, followed by detection of parasite burden by qPCR in the ascites of animals 7 days later ( n = 5). *** P < 0.001. e IFN-γ, IL-12p70, TNF-α and IL-10 in sera were tested by ELISA ( n = 5), *** P < 0.001, ** P < 0.01. f , g Toxoplasma -specific total IgG and subclasses of IgG1 and IgG2a in sera were tested by ELISA ( n = 10). Unpaired t -tests were used for statistical analysis. Bars = mean ± SEM. *** P < 0.001.

Article Snippet: In the meantime, the expression levels of cytokines interferon-gamma (IFN-γ), interleukin 12p70 (IL-12p70), tumor necrosis factor TNF (TNF-α) and interleukin 10 (IL-10) were detected by using ELISA kits (ProteinTech Group, Inc., USA) according to the manufacturer’s recommendations.

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: IL-12 cytokine levels in CSU patients and controls.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques: Control

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: IL-12 cytokine levels in CSU in the pre-therapy phase according to the period between disease onset and blood sampling.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques: Sampling

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: IL-12 cytokine levels in CSU in the active phase according to CRP values.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques:

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: IL-12 levels in CSU in active phase according to UAS7 score.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques:

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: Correlation between IL-12 variants and UAS7 and CRP, respectively, in CSU patients.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques:

Journal: Journal of Personalized Medicine

Article Title: Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria

doi: 10.3390/jpm14030295

Figure Lengend Snippet: Multifaceted IL-12 (IL-12p70, IL-12p35, IL-12p40) in CSU progression.

Article Snippet: ELISA kits were used to evaluate the quantitative systemic levels of IL-12p70, IL-12p35, and IL-12p40 in the study participants: Human IL-12p70 (Interleukin 12p70, IL12A, and IL12B heterodimer, disulfide-linked) ELISA Kit from ELABSCIENCE, Cat.No.

Techniques: Activation Assay, Activity Assay, Binding Assay, Mutagenesis